3 edition of The Binding and Transport of Anions in Living Tissue found in the catalog.
The Binding and Transport of Anions in Living Tissue
May 31, 1985
by Cambridge University Press
Written in English
Royal Society Discussion Volumes
|The Physical Object|
|Number of Pages||250|
This protein is found primarily in the liver and kidneys, with small amounts in other tissues such as the skin, stomach, blood vessels, and eyes. The MRP6 protein belongs to a group of proteins that transport molecules across cell membranes; however, little is known about the substances transported by MRP6. 4. Methods for measuring anion transport. The techniques used to detect and quantify anion transport fall into two categories: (a) methods employing vesicle suspensions, in which transport in and out of the vesicles is examined, and (b) conductance measurements on patches of synthetic or natural membranes, whole cells or sheets of tissue.
Berk PD, Potter BJ, Sorrentino D, Stremmel W, Stump D, Kiang CL, Zhou SL () Characteristics of organic anion binding proteins from rat liver sinusoidal plasma membranes. In: Petzinger E, Kinne RKH, Sies H (eds) Hepatic transport of organic substances. . ABCE1 is the single member in this family which is an organic anion-binding protein (its trivial name is OABP), sometimes confused with the 11 SLCO genes that encode solute-carrier organic anion transporters. ABCE1 has an ATP-binding domain but lacks the transmembrane domain, making it unlikely that this protein functions as a transporter.
Why Chloride Is Important For The Transport Of Negative Anions Across The Cell Membrane Words 7 Pages Chloride channels are a structurally diverse superfamily of transmembrane proteins that facilitate the transport of negative anions across the cell membrane. Gated ion transport across biological membranes is an intrinsic process regulated by protein channels. Synthetic anion carriers (anionophores) have potential applications in biological research; however, previously reported examples are mostly nonspecific, capable of mediating both electrogenic and electroneutral (nonelectrogenic) transport processes.
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Get this from a library. The Binding and transport of anions in living tissues: proceedings of a Royal Society discussion meeting held on 12 and 13 May, [R D Keynes; J C Ellory; Royal Society (Great Britain);]. 1. Philos Trans R Soc Lond B Biol Sci. Dec 1;() The binding and transport of anions in living tissues.
[No authors listed]. Abstract. Twenty years ago a book such as this would probably not have included a chapter on anion transport. At that time, there was little interest in anions, since it was felt that most important physiological processes involved transport of cations, and that anions simply went along with cations to maintain by: The Binding and Transport of Anions in Living Tissues.
Philos Trans R Soc (London) B - d depend on continuous experiments that are required to refine, or perhaps even discard the simple Michaelis - Menten kinetics model so well applied to Cited by: 7. The purpose was to determine the direction of organic anion (OA) transport across the ciliary body and the transport proteins that may contribute.
triphosphatase binding cassette families have. Binding and Transport Assays. Oocytes were divided into experimental groups of 16–20 and placed in a well plate with 1 ml of Barth's buffer containing a fluorescent anion tracer with a final concentration of 30 μ m 6-carboxyfluorescein for mOat1-injected oocytes, 50 μ m 5CF (mOat3), or 30 μ m fluorescein (mOat6), as well as various concentrations of unlabeled organic cations.
genomes of living organisms, like in E. coli, to which 10% of the genome belongs to transport rather must be appreciated as a metabolic cost, critical for cell vitality, especially when this. The binding activity was inhibited by the anion transport inhibitor DIDS and substrate analogs like succinate and oxamide, while EGTA or ruthenium red had no effect on binding, suggesting that the protein binding was oxalate site specific.
The molecular weight of the CaOx binding protein of different tissues was similar to that of renal cells. Anion transport studies The anion transport activities of the derivatives of 1 were determined in 1-palmitoyloleoyl-sn-glycerophosphocholine large unilamellar vesicles (POPC LUVs, lipid concentration 31 μM), loaded with 8-hydroxypyrene-1,3,6-trisulfonate (HPTS) buffered to pH in NaCl solution.
A pH gradient was applied across the. Ion transport. Movement of salts and other electrolytes in the form of ions from place to place within living systems. Ion transport may occur by any of several different mechanisms: electrochemical diffusion, active-transport requiring energy, or bulk flow as in the flow of blood in the circulatory system of animals or the transpiration stream in the xylem tissue of plants.
Everett A. Jenne, (Retired), in Adsorption of Metals by Geomedia, Anion Effect. Polyvalent anions (e.g., PO 4) readily form(Sposito ) noted that the increased metal adsorption sometimes observed after a strongly adsorbing anion has been reacted with a hydrous oxide adsorbent may be modeled either as a metal-anion surface precipitate effect or as metal-anion surface.
Organic anion-transporting polypeptide 1A2 (OATP1A2) (gene symbol, SLCO1A2) mediates cellular uptake of a wide range of endogenous substrates, as well as drugs and xenobiotics.
OATP1A2 is expressed in several tissues, including apical membranes of small intestinal epithelial cells. Given its role in intestinal drug absorption, a detailed analysis of the mechanisms that regulate SLCO1A2 gene.
Intracellular anion concentrations were estimated from anion-induced changes in cellular fluorescence using the measured affinity of purified YFP–HQ/IL ().The limit of detection of intracellular anions, defined as the concentration producing a fluorescence decrease of 3 times the standard deviation of baseline fluorescence (3 × SD = %, n = ), was 9 μM for perrhenate, 27.
Defective anion transport is a hallmark of the genetic disease cystic fibrosis (CF). One approach to restore anion transport to CF cells utilises alternative pathways for transmembrane anion transport, including artificial anion carriers (anionophores).
Here, we screened 22 anionophores for biological activi Chemical Science HOT Article Collection ChemSci Pick of the Week Collection. This review covers: 1) the tissue, cellular, and subcellular expression of drug transporters in the iris-ciliary body; 2) in vitro evidence for drug transport activity in the iris-ciliary body (with a focus on organic anions); and 3) in vivo evidence for the involvement of active transport in influencing intraocular drug concentrations, with a.
Anions bind to the cavity between the β-chains of the T-state in tetrameric hemoglobins. Hagfish and lampreys are devoid of anion effects, because their hemoglobins do not form stable tetramers.
The effectiveness of binding increases with the number of (hydrogen) bonds that the compound can form with the hemoglobin.
By studying anion binding affinity and transport mechanisms of four other anion transporters, we show that the lack of uniport and the voltage‐dependent H+/Cl− symport originate from strong. Monien B. et al. Probenecid, an inhibitor of transmembrane organic anion transporters, alters tissue distribution of DNA adducts in 1-hydroxymethylpyrene-treated rats.
Toxicology80–5 (). Enomoto A. et al. Interaction of human organic anion transporters 2 and 4 with organic anion transport inhibitors. Pharmacol.
Exp. Anion binding to this site also controls the electromotile activity of prestin. CBE subserves the systemic transport of CO2 from tissues to lungs, while in other cells, CBE activity might be. Calcium ions (Ca 2+) contribute to the physiology and biochemistry of organisms play an important role in signal transduction pathways, where they act as a second messenger, in neurotransmitter release from neurons, in contraction of all muscle cell types, and in enzymes require calcium ions as a cofactor, including several of the coagulation factors.
Introduction. Biologically-active synthetic transmembrane anion transporters (anionophores) have therapeutic potential in the genetic disease cystic fibrosis (CF).1–5 CF is caused by dysfunction of the anion channel cystic fibrosis transmembrane conductance regulator (CFTR), which plays a pivotal role in salt and water transport across epithelia.6–8 Although some drugs have been .The anion binding and transport properties of an extensive library of thiophene‐based molecules are reported.
Seventeen bis‐urea positional isomers, with different binding conformations and.Questions and answers on brain tissue binding. Please provide an overview of Cyprotex's Brain Tissue Binding assay. Equilibrium dialysis is used to determine the extent of binding of a compound to brain tissue.
A semi-permeable membrane separates a compartment containing compound in brain homogenate from a compartment containing compound in buffer.